Without the presence of donor template, random repair through the non-homologous end joining (NHEJ) pathway usually results in a frameshift of the gene coding sequence. sgRNAs targeting specific loci in the genome guide Cas9 enzyme to the locus of interest, where Cas9 nuclease cuts the target site.

Synthetic sgRNAs and Cas9 mRNA are co-injected into mouse fertilized eggs. It usually takes 4-6 months to generate a conventional (constitutive) gene knockout mouse model via CRISPR gene editing technology. Generate a conventional (constitutive) gene knockout (KO) mouse model to permanently inactivate your target gene. Check out our website to find out whether your model of interest is available for direct purchase.Īlternatively, you may contact our technical support staff to design a customized, gene knock-out model that fits your need best.Ĭonventional (Constitutive) Gene Knockout Shanghai Model Organisms Center (SMOC) has more than 6000 research-ready GEM models. Simplify the workflow of drug candidate evaluationĬonventional (constitutive) gene knockout

Gain an in-depth understanding of the etiology of human diseases

Uncover gene functions and explore cellular processes Accelerate your research with customized gene knockout mouse models that provide thorough insight into key genetic mechanisms.

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